Cross Reactivities of Rabbit Anti-Chicken Horse Radish Peroxidase Conjugate with Sera of Some other Avian Species in ELISA System

ed by: African Index Medicus (WHO), CAB Abstracts, Index Copernicus, Global Health Abstracts, Asian Science Index, Index Veterinarius, Bioline International , African Journals online Full-text available at http://www.ajbrui.com http://www.bioline.br/md http://www.ajol.com Received: Februaru 2007 Accepted (Revised): April 2007 Published May 2007 Short communication Cross Reactivities of Rabbit Anti-Chicken Horse Radish Peroxidase Conjugate with Sera of Some other Avian Species in ELISA System Owoade A. A.; Fagbohun O.A. and Oluwayelu D. O. Faculty of Veterinary Medicine, University of Ibadan, Ibadan. Nigeria. ABSTRACT The cross reactivities of rabbit anti chicken horse radish peroxidase (conjugate) was tested with sera of Chicken, Ducks, Geese, Guinea fowl, Hawks, Pigeons and Turkeys in indirect enzyme linked immunosorbent assay (ELISA) technique. Sera from mammalian species (Bat, Equine and swine) were used as negative controls. The conjugate was coated on wells of ELISA micro titer plate and sera from the avian and mammalian species added. Reactivity was detected with OPD. There were losses of reactivities when sera were diluted as from 1\78125. Chicken and Turkey sera reacted with the conjugate without loss of reactivities when either normal rabbit serum (NRS) or bovine serum albumin (BSA) was used as blocking agent. Sera of other avian species and mammals did not react with the conjugate. It is concluded that rabbit anti chicken Horse radish peroxidase could be used to detect antibodies in chickens as well as Turkey and that BSA and NRS could be used as blocking agent without loss of reactivities. (Afr. J. Biomed. Res. 10: 193 196)The cross reactivities of rabbit anti chicken horse radish peroxidase (conjugate) was tested with sera of Chicken, Ducks, Geese, Guinea fowl, Hawks, Pigeons and Turkeys in indirect enzyme linked immunosorbent assay (ELISA) technique. Sera from mammalian species (Bat, Equine and swine) were used as negative controls. The conjugate was coated on wells of ELISA micro titer plate and sera from the avian and mammalian species added. Reactivity was detected with OPD. There were losses of reactivities when sera were diluted as from 1\78125. Chicken and Turkey sera reacted with the conjugate without loss of reactivities when either normal rabbit serum (NRS) or bovine serum albumin (BSA) was used as blocking agent. Sera of other avian species and mammals did not react with the conjugate. It is concluded that rabbit anti chicken Horse radish peroxidase could be used to detect antibodies in chickens as well as Turkey and that BSA and NRS could be used as blocking agent without loss of reactivities. (Afr. J. Biomed. Res. 10: 193 196)